Molecular Mechanism of Target RNA Transcript Recognition by Argonaute-Guide Complexes
نویسندگان
چکیده
منابع مشابه
Structural basis for the recognition of guide RNA and target DNA heteroduplex by Argonaute
Argonaute proteins are key players in the gene silencing mechanisms mediated by small nucleic acids in all domains of life from bacteria to eukaryotes. However, little is known about the Argonaute protein that recognizes guide RNA/target DNA. Here, we determine the 2 Å crystal structure of Rhodobacter sphaeroides Argonaute (RsAgo) in a complex with 18-nucleotide guide RNA and its complementary ...
متن کاملAssembly and analysis of eukaryotic Argonaute–RNA complexes in microRNA-target recognition
Experimental studies have uncovered a variety of microRNA (miRNA)-target duplex structures that include perfect, imperfect and seedless duplexes. However, non-canonical binding modes from imperfect/seedless duplexes are not well predicted by computational approaches, which rely primarily on sequence and secondary structural features, nor have their tertiary structures been characterized because...
متن کاملDNA recognition by an RNA-guided bacterial Argonaute
Argonaute (Ago) proteins are widespread in prokaryotes and eukaryotes and share a four-domain architecture capable of RNA- or DNA-guided nucleic acid recognition. Previous studies identified a prokaryotic Argonaute protein from the eubacterium Marinitoga piezophila (MpAgo), which binds preferentially to 5'-hydroxylated guide RNAs and cleaves single-stranded RNA (ssRNA) and DNA (ssDNA) targets. ...
متن کاملCharacterization of protein and small RNA components of Argonaute complexes
Diese Dissertation wurde selbständig, ohne unerlaubte Hilfe erarbeitet. Summary Members of the Argonaute (Ago) protein family associate with small RNAs and regulate gene expression at the level of transcription, mRNA stability or translation. Although a number of small RNAs and proteins involved in gene silencing have been identified so far, the spectrum of Ago-mediated silencing implies an int...
متن کاملA bacterial Argonaute with noncanonical guide RNA specificity.
Eukaryotic Argonaute proteins induce gene silencing by small RNA-guided recognition and cleavage of mRNA targets. Although structural similarities between human and prokaryotic Argonautes are consistent with shared mechanistic properties, sequence and structure-based alignments suggested that Argonautes encoded within CRISPR-cas [clustered regularly interspaced short palindromic repeats (CRISPR...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Symposia on Quantitative Biology
سال: 2006
ISSN: 0091-7451,1943-4456
DOI: 10.1101/sqb.2006.71.029